t cell jurkat batch (Eppendorf AG)
Structured Review

T Cell Jurkat Batch, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 191818 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+cell+jurkat+batch/pmc13093239-1-0-5?v=Eppendorf+AG
Average 99 stars, based on 191818 article reviews
Images
1) Product Images from "Capacitance Technology Enables Automated Feeding, Improved Expansion, and Higher Throughput of CAR‐T Cell Stirred‐Tank Bioreactor Cultures"
Article Title: Capacitance Technology Enables Automated Feeding, Improved Expansion, and Higher Throughput of CAR‐T Cell Stirred‐Tank Bioreactor Cultures
Journal: Biotechnology Journal
doi: 10.1002/biot.70226
Figure Legend Snippet: Outputs of capacitance measurements for a T cell (Jurkat) bolus fed process. Panel (A) overlays the online capacitance trace for the LBB process with the viable and total cell concentrations. Feed points have also been marked on the figure. Panel (B) reproduces the lag phase data (the highlighted region in A) for the run to demonstrate the probe's ability to produce correlative results well below the typically observed operational ranges of the probes seen in current biotechnology processes. Panel (C) is a bubble‐plot of viable cell concentration versus capacitance which displays cell diameter and overall culture viability.
Techniques Used: Concentration Assay
Figure Legend Snippet: Summary data for various feeding regimes employed in T cell (Jurkat) cultures. Panel (A) summarizes the different feeding regimes with respect to process time used for the various Jurkat runs. Panels B‐F all present the graphs of sample time recorded capacitance, viable cell number and culture volume (for ease of understanding feeding implications). Feed zones are marked by the shaded region and relevant detail given in magenta text. Panel (B) = batch process; Panel (C) = LBB; Panel (D) = capacitance‐based bolus; Panel (E) = linear feed; Panel (F) = exponential Feed.
Techniques Used:
Figure Legend Snippet: Summary data for the different feed regime processes for T cells (Jurkat). Panel (A) gives an overlaid viable cell number graph for the different processes to compare culture time and cell yields obtained across the processes. Panel (B) presents the viability data for the same processes to allow for understanding of where ideal harvest points may occur. Panel (C) contextualizes the harvest point yields (selected as last point of ideal viability) in terms of largest doses of Kymriah for the treatment of either ALL or B‐cell lymphoma.
Techniques Used: